کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2024752 | 1542615 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Possible contribution of alternative nitrogenases to nitrogen fixation by asymbiotic N2-fixing bacteria in soils
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش خاک شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Possible contribution of alternative nitrogenases to nitrogen fixation by asymbiotic N2-fixing bacteria in soils Possible contribution of alternative nitrogenases to nitrogen fixation by asymbiotic N2-fixing bacteria in soils](/preview/png/2024752.png)
چکیده انگلیسی
The relative contributions of the alternative (V- and Fe-only) and canonical (Mo-) nitrogenases to N2 fixation in natural environments are still largely unknown. Here we combine chemical and molecular approaches to examine their role in terrestrial ecosystems. Pure-culture studies show that while the R ratio (defined as RÂ =Â acetylene reduction rate/N2 fixation rate by nitrogenase) is highly variable, low R ratios (0.5Â <Â RÂ <Â 2) are usually an indication of alternative nitrogenase activity. Microcosm experiments with temperate soils showed low R ratios and a large increase in N2 fixation rates in response to vanadium amendments, indicating V-nitrogenase activity. Using RT-PCR, we were able to demonstrate expression of the V-nitrogenase, together with the Mo-nitrogenase, in these soils. An analysis of published R ratios showed that the contribution of alternative nitrogenases to asymbiotic N2 fixation in soils may be more prevalent than previously recognized.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 69, February 2014, Pages 413-420
Journal: Soil Biology and Biochemistry - Volume 69, February 2014, Pages 413-420
نویسندگان
J.P. Bellenger, Y. Xu, X. Zhang, F.M.M. Morel, A.M.L. Kraepiel,