An evaluation of the functional significance of peat microorganisms using a reciprocal transplant approach

Our current knowledge on the relevance of microbial diversity and composition for the recovery and maintenance of soil biological processes is rudimentary, partly because experimental substantiation of the importance of community composition to function is scarce. Guided by this gap, we devised a reciprocal transplant experiment to examine the functional behaviour of different microbial communities exposed to two structurally distinct peats. Sterile peat samples representing two types, one humified (sedge) and the other dominated by coarse plant material (fibric), were inoculated with a 10−1, 10−3, 10−5, or 10−8 dilution of either the same or reciprocal peats. After 5 months of incubation, we used a nucleotide-analog technique to label the active bacterial taxa in samples receiving the 10−1 and 10−8 dilutions. We assessed both the peats' functional potential (respiration and nutrient-acquiring and lignin-degrading enzyme activities) and the structures of active and total bacterial communities (PCR-DGGE). In general, we found a decline in respiration rates and increase in enzyme activities with increasing dilution level, but the effect of dilution on bacterial richness was weak. The bacterial community structure and richness depended on both the inoculum source and the peat type. The activity of enzymes involved in nutrient acquisition depended mainly on the soil type, while the lignin-degrading activity was influenced by differences in community composition between peat types. Neither active bacterial populations nor respiration were significantly influenced by peat type or inoculum source. Our results suggest that the relationship between microbial community composition and function is not only related to the taxonomic breadth of the taxa that perform a given function, but may also be shaped by interactions between microorganisms in the inoculum source and the substrate being colonized.