کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2025348 | 1069992 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of loop-mediated isothermal amplification (LAMP) to rapidly detect arbuscular mycorrhizal fungi
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش خاک شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of loop-mediated isothermal amplification (LAMP) to rapidly detect arbuscular mycorrhizal fungi Evaluation of loop-mediated isothermal amplification (LAMP) to rapidly detect arbuscular mycorrhizal fungi](/preview/png/2025348.png)
چکیده انگلیسی
Detection and quantification of arbuscular mycorrhizal fungi (AMF) is important for any work with this group of root symbionts. We here applied loop-mediated amplification (LAMP), an isothermal DNA amplification technique, to AMF under in vitro culture conditions. In this first application of LAMP to AMF, we introduce LAMP primers that were able to amplify AMF DNA from colonized carrot roots (Glomus intraradices), and genomic DNA from several taxa of AMF (input template as low as 1×10−2 ng). This study is the starting point for testing this method under more challenging environmental conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 40, Issue 2, February 2008, Pages 540–543
Journal: Soil Biology and Biochemistry - Volume 40, Issue 2, February 2008, Pages 540–543
نویسندگان
Vijay Gadkar, Matthias C. Rillig,