کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2026239 1070023 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Potential importance of bacteria and fungi in nitrate assimilation in soil
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
پیش نمایش صفحه اول مقاله
Potential importance of bacteria and fungi in nitrate assimilation in soil
چکیده انگلیسی

Soil microorganisms can use a wide range of N compounds but are thought to prefer NH4+. Nevertheless, 15N isotope dilution studies have shown that microbial immobilization of NO3− can be an important process in many soils, particularly relatively undisturbed soils. Our objective was to develop a method for measuring NO3− immobilization potential so that the relative contributions of bacteria and fungi could be determined. We modified and optimized a soil slurry method that included amendments of KNO3, glucose, and methionine sulfoximine (an inhibitor of N assimilation) in the presence of two protein synthesis inhibitors: chloramphenicol, which inhibits bacteria, or cycloheximide, which inhibits fungi. By adding 15N-labeled KNO3, we were able to measure gross rates of NO3− production (i.e., gross nitrification) and consumption (i.e., gross NO3− immobilization). We found that bacteria, not fungi, had the greatest potential for assimilating, or immobilizing, NO3− in these soils. This is consistent with their growth habit and distribution in the heterogeneous soil matrix.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 39, Issue 7, July 2007, Pages 1737–1743
نویسندگان
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