کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2026281 | 1070024 | 2010 | 4 صفحه PDF | دانلود رایگان |
A silica-based solid phase extraction (SPE) protocol commonly used to isolate phospholipids from total lipid extracts failed to quantitatively recover phosphatidylcholines (PC) from three commercial SPE columns because a polar eluent volume of 5 mL methanol per 0.5 g silica was shown to be insufficient. Phosphatidylcholines, which are an important component of some fungal and bacterial cell membranes, were completely recovered when a larger ratio of 20:1 v/w methanol (mL) to silica (g) was used. Separation of phospholipids from a soil sample showed that a methanol:silica ratio of 20:1 recovered substantially greater amounts of phospholipids and resulted in a different phospholipid fatty acid (PLFA) structural profile than a 10:1 ratio. This study also confirmed that methanol preconditioning of the manufactured SPE columns studied is necessary for quantitative recovery of phospholipids.
Journal: Soil Biology and Biochemistry - Volume 42, Issue 7, July 2010, Pages 1179–1182