کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2027717 1542704 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of natural and synthetic glucocorticoids in calf urine following different growth-promoting prednisolone treatments
ترجمه فارسی عنوان
اندازه گیری گلوکوکورتیکویدها طبیعی و مصنوعی در ادرار گوسفند پس از درمان های مختلف پردنیزولون با رشد
کلمات کلیدی
پیشینسولون، دگزامتازون، 6 ²-هیدروکسی هیدروکورتیزون، ادرار گاو، استفاده غیرقانونی،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Over the last few years, low levels of prednisolone have been reported in several cattle urine samples by a number of laboratories within the EU at an average concentration of 2.0 ng mL−1. The occurrence of prednisolone residues together with increased levels of hydrocortisone and cortisone in urine and tissue samples of untreated animals seems to demonstrate that traces of this steroid can be produced endogenously during stressful situations. Therefore, the endogenous origin of prednisolone makes difficult to correlate positive samples to a potential illicit treatment. An experimental study was developed to investigate the presence of natural and synthetic glucocorticoids and to evaluate levels of excreted prednisolone following growth-promoting treatments. Urine samples from calves undergone oral treatment with prednisolone, alone and in association with dexamethasone, were analyzed by a LC-MS/MS method, validated according to the Commission Decision 2002/657/EC. We also investigated if urinary free 6β-hydroxyhydrocortisone/hydrocortisone ratio could be a reliable biomarker of illicit treatment with prednisolone and dexamethasone in calves. Our data revealed that urinary levels of prednisolone after both oral prednisolone treatments, never exceeded the value of 1.1 ng mL−1. Similar prednisolone levels were found in urine samples of untreated calves. Moreover the presence of 6β-hydroxyhydrocortisone below the CCα value made possible to estimate the 6β-hydroxyhydrocortisone/hydrocortisone ratio only in a very limited number of samples. Obtained data suggest that further criteria have to be considered to allow correct decisions about the urinary presence of prednisolone during control activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Steroids - Volume 104, December 2015, Pages 196-202
نویسندگان
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