Differential metabolism of androst-5-ene-3β,17β-diol between rats, canines, monkeys and humans

► The majority of rat 5-AED metabolites contained three or more oxygen substituents. ► Human hepatocytes primarily metabolized 5-AED into DHEA. ► Very little 5-AED and DHEA were hydroxylated by human hepatocytes. ► Humans do not significantly metabolize DHEA into anti-inflammatory triols.