کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2029041 | 1542744 | 2006 | 11 صفحه PDF | دانلود رایگان |
BackgroundThere is a growing awareness that oxidative stress may play a role in periodontal disease. The aim of this investigation was to evaluate potential oxidant/antioxidant interactions of nicotine with antioxidants (Coenzyme Q10 (CoQ), Pycnogenol®) and phytoestrogens in a cell culture model.MethodsDuplicate incubations of human periosteal fibroblasts and osteoblasts were performed with 14C-testosterone as substrate, in the presence or absence of CoQ (20 μg/ml), Pycnogenol® (150 μg/ml), and phytoestrogens (10 and 40 μg/ml), alone and in combination with nicotine (250 μg/ml). At the end of a 24-h incubation period, the medium was solvent extracted and testosterone metabolites were separated by thin-layer chromatography and quantified using a radioisotope scanner.ResultsThe incubations of osteoblasts and periosteal fibroblasts with CoQ, Pycnogenol® or phytoestrogens stimulated the synthesis of the physiologically active androgen DHT, while the yields of DHT were significantly reduced in response to nicotine compared to control values (p < 0.001 for phytoestrogens). The combination of nicotine with CoQ, Pycnogenol® or phytoestrogens increased the yields of DHT compared with incubation with nicotine alone in both cell types.ConclusionThis investigation suggests that the catabolic effects of nicotine could be reversed by the addition of antioxidants such as CoQ or Pycnogenol® and phytoestrogens.
Journal: Steroids - Volume 71, Issues 13–14, December 2006, Pages 1062–1072