Two novel C29-5β-sterols from the stems of Opuntia dillenii

Two novel C29-5β-sterols, opuntisterol [(24R)-24-ethyl-5β-cholest-9-ene-6β,12α-diol] (1) and opuntisteroside [(24R)-24-ethyl-6β-[(β-d-glucopyranosyl)oxy]-5β-cholest-9-ene-12α-ol] (2), together with nine known compounds, β-sitosterol (3), taraxerol (4), friedelin (5), methyl linoleate (6), 7-oxositosterol (7), 6β-hydroxystigmast-4-ene-3-one (8), daucosterol (9), methyl eucomate (10) and eucomic acid (11), were isolated from the stems of Opuntia dillenii collected in Guizhou Province, China. Their structures were elucidated mainly by spectroscopic analysis. The absolute configuration of 1 were deduced from comparative 1H NMR data of the (S)- and (R)-methoxyphenyl acetate derivatives. Compounds 6–8, 10 and 11 were isolated from O. dillenii for the first time.