کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2033963 1071975 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New immunization protocol to produce crotalic antivenom combining Crotalus durissus terrificus venom and its PLA2
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
New immunization protocol to produce crotalic antivenom combining Crotalus durissus terrificus venom and its PLA2
چکیده انگلیسی


• This work proposes an alternative protocol to produce crotalic antivenom (MS).
• We designed an immunization plan using isolated crotalic PLA2 and venom injections.
• The MS produced has better efficacy than those produced by conventional protocols.

Antivenoms are usually obtained by animal immunization with successive inoculations of increasing sublethal amounts of venom, which may impair the animal health. The high lethality of venom requires prolonged immunization plans with small amounts of venom. Thus, we propose an alternative plan that includes a pre-immunization of the animal with phospholipase A2, the main crotoxin component, which is responsible for the whole venom lethality. For comparison, three different immunization schemes were designed: high dose protocol (HDP; 0.5–27 mg of venom), low dose protocol (LDP; 0.1–7 mg of venom) and Mix protocol (MP; preimmunization 0.1–1.2 mg of crotalic PLA2, and then 4.5–8 mg of venom). Antibody titers were determined by ELISA, in blood plasma obtained from the marginal vein of the ear. The neutralizing ability of the different sera obtained by all protocols (HDS, LDS and MS) was tested against the most important pharmacological activities of whole venom: PLA2 activity, myotoxicity, thrombin like activity and lethality. MS showed the best neutralizing efficacy and at the same time, it was obtained by an immunization protocol that takes account of animal health care, since it requires low quantities of venoms in comparison to traditional protocols.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 43, Issue 1, January 2015, Pages 62–70
نویسندگان
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