کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2033990 | 1071978 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fat harvesting site is an important determinant of proliferation and pluripotency of adipose-derived stem cells
ترجمه فارسی عنوان
سایت برداشت چربی عامل مهمی در گسترش و تکثیر سلول های بنیادی حاصل از چربی است
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی
To define the optimal fat harvest site and detect any potential differences in adipose-derived stem cells (ASCs) proliferation properties in camels, aspirates from the abdomen and hump sites were compared. Obtained results revealed that ASCs from both abdomen and hump exhibited spindle-shaped and fibroblast-like morphology with hump-derived ASCs being smaller in size and narrower in overall appearance than abdominal ASCs. Abdominal ASCs required a greater time for proliferation than the hump-derived cells. These results were further confirmed with a tetrazolium-based colorimetric assay (MTT) which showed a greater cell proliferation rate for hump ASCs than for the abdomen. Under inductive conditions, ASCs from both abdominal and hump fat deposits maintained their lineage differentiation potential into adipogenic, chondrogenic, and osteogenic lineages during subsequent passages without any qualitative difference. However, expression of alkaline phosphatase was higher in osteogenic differentiated cells from the hump compared with those of the abdomen. Moreover, the increase in calcium content in hump-derived stem cells was higher than that in abdominal-derived stem cells. In conclusion, our findings revealed that ASCs can be obtained from different anatomical locations, although ASCs from the hump fat region may be the ideal stem cell sources for use in cell-based therapies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 44, Issue 1, January 2016, Pages 12-18
Journal: Biologicals - Volume 44, Issue 1, January 2016, Pages 12-18
نویسندگان
Abdolreza Ardeshirylajimi, Farjad Rafeie, Ali Zandi-Karimi, Ghobad Asgari Jaffarabadi, Abdollah Mohammadi-Sangcheshmeh, Rahmat Samiei, Abdolhakim Toghdory, Ehsan Seyedjafari, Seyed Mahmoud Hashemi, Mehmet Ulas Cinar, Eduardo L. Gastal,