کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2034352 1072008 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro antibody response to tetanus in the MIMIC™ system is a representative measure of vaccine immunogenicity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
In vitro antibody response to tetanus in the MIMIC™ system is a representative measure of vaccine immunogenicity
چکیده انگلیسی

In response to the recurrent failure of animal vaccine protection studies to accurately predict human trial results, we have developed a fully human modular immune in vitroconstruct (MIMIC™) to serve as a preliminary screen for efficacy testing of potential vaccine formulations. To validate the potential of this approach, we monitored the in vitro-generated tetanus (TT)-specific antibody levels in a cohort of donors before and after receiving tetanus vaccination. Purified CD4_T cell and B cell populations were combined with autologous tetanus vaccine-pulsed dendritic cells to generate specific antibody. Enumeration of TT-specific IgG antibody-secreting cells by ELISPOT displayed a significant increase in the magnitude of this population after vaccination. The relative magnitudes of the in vitro-generated TT-specific antibody response before and after vaccination largely recapitulated the TT-specific IgG serum titer profiles measured in the same individuals. These findings provide evidence that the MIMIC™ system can be a rapid and representative in vitro method for measuring vaccine immunogenicity via induction of the memory B cell response.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 37, Issue 3, June 2009, Pages 148–151
نویسندگان
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