کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2035345 1543093 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The RNA Binding Protein Quaking Regulates Formation of circRNAs
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
The RNA Binding Protein Quaking Regulates Formation of circRNAs
چکیده انگلیسی


• Numerous circRNAs are regulated during epithelial to mesenchymal transition
• Quaking (QKI) regulates circRNA formation during EMT
• QKI binds to sites flanking circRNA-forming exons
• Insertion of QKI binding sites into linear RNA can induce exon circularization

SummaryCircular RNAs (circRNAs), formed by non-sequential back-splicing of pre-mRNA transcripts, are a widespread form of non-coding RNA in animal cells. However, it is unclear whether the majority of circRNAs represent splicing by-products without function or are produced in a regulated manner to carry out specific cellular functions. We show that hundreds of circRNAs are regulated during human epithelial-mesenchymal transition (EMT) and find that the production of over one-third of abundant circRNAs is dynamically regulated by the alternative splicing factor, Quaking (QKI), which itself is regulated during EMT. Furthermore, by modulating QKI levels, we show the effect on circRNA abundance is dependent on intronic QKI binding motifs. Critically, the addition of QKI motifs is sufficient to induce de novo circRNA formation from transcripts that are normally linearly spliced. These findings demonstrate circRNAs are both purposefully synthesized and regulated by cell-type specific mechanisms, suggesting they play specific biological roles in EMT.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 160, Issue 6, 12 March 2015, Pages 1125–1134
نویسندگان
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