کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2035372 | 1072163 | 2014 | 14 صفحه PDF | دانلود رایگان |
• Identification of a protein complex that antagonizes Cdc42 activation is discussed
• Cdc42 inhibitory complexes stably associate with cytokinesis remnants
• Cytokinesis remnants act as memory for previous polarization events
• Negative polarity cues at cytokinesis remnants prevent replicative aging
SummaryCdc42 is a highly conserved master regulator of cell polarity. Here, we investigated the mechanism by which yeast cells never re-establish polarity at cortical sites (cytokinesis remnants [CRMs]) that have previously supported Cdc42-mediated growth as a paradigm to mechanistically understand how Cdc42-inhibitory polarity cues are established. We revealed a two-step mechanism of loading the Cdc42 antagonist Nba1 into CRMs to mark these compartments as refractory for a second round of Cdc42 activation. Our data indicate that Nba1 together with a cortically tethered adaptor protein confers memory of previous polarization events to translate this spatial legacy into a biochemical signal that ensures the local singularity of Cdc42 activation. “Memory loss” mutants that repeatedly use the same polarity site over multiple generations display nuclear segregation defects and a shorter lifespan. Our work thus established CRMs as negative polarity cues that prevent Cdc42 reactivation to sustain the fitness of replicating cells.
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Journal: - Volume 159, Issue 5, 20 November 2014, Pages 1056–1069