Multifocal Epithelial Tumors and Field Cancerization from Loss of Mesenchymal CSL Signaling

SummaryIt is currently unclear whether tissue changes surrounding multifocal epithelial tumors are a cause or consequence of cancer. Here, we provide evidence that loss of mesenchymal Notch/CSL signaling causes tissue alterations, including stromal atrophy and inflammation, which precede and are potent triggers for epithelial tumors. Mice carrying a mesenchymal-specific deletion of CSL/RBP-Jκ, a key Notch effector, exhibit spontaneous multifocal keratinocyte tumors that develop after dermal atrophy and inflammation. CSL-deficient dermal fibroblasts promote increased tumor cell proliferation through upregulation of c-Jun and c-Fos expression and consequently higher levels of diffusible growth factors, inflammatory cytokines, and matrix-remodeling enzymes. In human skin samples, stromal fields adjacent to multifocal premalignant actinic keratosis lesions exhibit decreased Notch/CSL signaling and associated molecular changes. Importantly, these changes in gene expression are also induced by UVA, a known environmental cause of cutaneous field cancerization and skin cancer.

Graphical AbstractFigure optionsDownload high-quality image (228 K)Download as PowerPoint slideHighlights
► Mesenchymal loss of CSL/Notch results in field cancerization of the skin epithelium
► Protumorigenic consequences of CSL loss are linked to c-Jun/c-Fos upregulation
► Anti-inflammatory treatment counteracts the field cancerization phenotype
► UVA exposure alters DNA methylation to downregulate stromal Notch signaling