کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2035697 | 1072212 | 2012 | 12 صفحه PDF | دانلود رایگان |
SummaryTo accommodate the large cells following zygote formation, early blastomeres employ modified cell divisions. Karyomeres are one such modification, mitotic intermediates wherein individual chromatin masses are surrounded by nuclear envelope; the karyomeres then fuse to form a single mononucleus. We identified brambleberry, a maternal-effect zebrafish mutant that disrupts karyomere fusion, resulting in formation of multiple micronuclei. As karyomeres form, Brambleberry protein localizes to the nuclear envelope, with prominent puncta evident near karyomere-karyomere interfaces corresponding to membrane fusion sites. brambleberry corresponds to an unannotated gene with similarity to Kar5p, a protein that participates in nuclear fusion in yeast. We also demonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish. Our studies provide insight into the machinery required for karyomere fusion and suggest that specialized proteins are necessary for proper nuclear division in large dividing blastomeres.
Graphical AbstractFigure optionsDownload high-quality image (375 K)Download as PowerPoint slideHighlights
► Brambleberry (Bmb) promotes nuclear envelope fusion of karyomeres
► Bmb assembles into foci, marking future and active karyomere fusion site
► Loss of maternal Bmb causes multiple micronuclei to form in cleavage stage cells
► Bmb acts in pronuclear fusion during zygote formation following fertilization
Journal: - Volume 150, Issue 3, 3 August 2012, Pages 521–532