کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2036416 | 1072263 | 2010 | 13 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Recombination Proteins Mediate Meiotic Spatial Chromosome Organization and Pairing Recombination Proteins Mediate Meiotic Spatial Chromosome Organization and Pairing](/preview/png/2036416.png)
SummaryMeiotic chromosome pairing involves not only recognition of homology but also juxtaposition of entire chromosomes in a topologically regular way. Analysis of filamentous fungus Sordaria macrospora reveals that recombination proteins Mer3, Msh4, and Mlh1 play direct roles in all of these aspects, in advance of their known roles in recombination. Absence of Mer3 helicase results in interwoven chromosomes, thereby revealing the existence of features that specifically ensure “entanglement avoidance.” Entanglements that remain at zygotene, i.e., “interlockings,” require Mlh1 for resolution, likely to eliminate constraining recombinational connections. Patterns of Mer3 and Msh4 foci along aligned chromosomes show that the double-strand breaks mediating homologous alignment have spatially separated ends, one localized to each partner axis, and that pairing involves interference among developing interhomolog interactions. We propose that Mer3, Msh4, and Mlh1 execute all of these roles during pairing by modulating the state of nascent double-strand break/partner DNA contacts within axis-associated recombination complexes.
Graphical AbstractFigure optionsDownload high-quality image (407 K)Download as PowerPoint slideHighlights
► Recombination proteins Mer3, Msh4, and Mlh1 play an early role in meiotic pairing
► Msh4 determines alignment spacing of homologous chromosomes
► Homolog pairing involves precrossover interference and entanglement avoidance
► Mlh1p is required for postalignment resolution of interlocks
Journal: - Volume 141, Issue 1, 2 April 2010, Pages 94–106