کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2036483 1072267 2010 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biological and Molecular Heterogeneity of Breast Cancers Correlates with Their Cancer Stem Cell Content
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Biological and Molecular Heterogeneity of Breast Cancers Correlates with Their Cancer Stem Cell Content
چکیده انگلیسی

SummaryPathways that govern stem cell (SC) function are often subverted in cancer. Here, we report the isolation to near purity of human normal mammary SCs (hNMSCs), from cultured mammospheres, on the basis of their ability to retain the lipophilic dye PKH26 as a consequence of their quiescent nature. PKH26-positive cells possess all the characteristics of hNMSCs. The transcriptional profile of PKH26-positive cells (hNMSC signature) was able to predict biological and molecular features of breast cancers. By using markers of the hNMSC signature, we prospectively isolated SCs from the normal gland and from breast tumors. Poorly differentiated (G3) cancers displayed higher content of prospectively isolated cancer SCs (CSCs) than did well-differentiated (G1) cancers. By comparing G3 and G1 tumors in xenotransplantation experiments, we directly demonstrated that G3s are enriched in CSCs. Our data support the notion that the heterogeneous phenotypical and molecular traits of human breast cancers are a function of their CSC content.

Graphical AbstractFigure optionsDownload high-quality image (290 K)Download as PowerPoint slideHighlights
► Human mammary stem cells (hNMSCs) can be isolated to a high degree of purity
► hNMSCs have a distinctive molecular signature
► The signature distinguishes breast cancers of different grades and molecular subtypes
► Breast cancer stem cell content relates to degree of differentiation and prognosis

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 140, Issue 1, 8 January 2010, Pages 62–73
نویسندگان
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