کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2036487 | 1072267 | 2010 | 12 صفحه PDF | دانلود رایگان |
SummaryMicroRNAs (miRNAs) control gene expression in animals and plants. Like another class of small RNAs, siRNAs, they affect gene expression posttranscriptionally. While siRNAs in addition act in transcriptional gene silencing, a role of miRNAs in transcriptional regulation has been less clear. We show here that in moss Physcomitrella patens mutants without a DICER-LIKE1b gene, maturation of miRNAs is normal but cleavage of target RNAs is abolished and levels of these transcripts are drastically reduced. These mutants accumulate miRNA:target-RNA duplexes and show hypermethylation of the genes encoding target RNAs, leading to gene silencing. This pathway occurs also in the wild-type upon hormone treatment. We propose that initiation of epigenetic silencing by DNA methylation depends on the ratio of the miRNA and its target RNA.
Graphical SummaryFigure optionsDownload high-quality image (212 K)Download as PowerPoint slideHighlights
► The moss DICER-LIKE1a (PpDCL1a) protein is required for miRNA biogenesis
► The related PpDCL1b protein is required for target cleavage but not miRNA biogenesis
► In PpDCL1b mutants, genes encoding miRNA targets are silenced by DNA methylation
► This epigenetic gene silencing is initiated by high miRNA to target RNA ratios
Journal: - Volume 140, Issue 1, 8 January 2010, Pages 111–122