کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2036551 | 1072270 | 2010 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Actin Dynamics Drive Membrane Reorganization and Scission in Clathrin-Independent Endocytosis Actin Dynamics Drive Membrane Reorganization and Scission in Clathrin-Independent Endocytosis](/preview/png/2036551.png)
SummaryNascent transport intermediates detach from donor membranes by scission. This process can take place in the absence of dynamin, notably in clathrin-independent endocytosis, by mechanisms that are yet poorly defined. We show here that in cells scission of Shiga toxin-induced tubular endocytic membrane invaginations is preceded by cholesterol-dependent membrane reorganization and correlates with the formation of membrane domains on model membranes, suggesting that domain boundary forces are driving tubule membrane constriction. Actin triggers scission by inducing such membrane reorganization process. Tubule occurrence is indeed increased upon cellular depletion of the actin nucleator component Arp2, and the formation of a cortical actin shell in liposomes is sufficient to trigger the scission of Shiga toxin-induced tubules in a cholesterol-dependent but dynamin-independent manner. Our study suggests that membranes in tubular Shiga toxin-induced invaginations are poised to undergo actin-triggered reorganization leading to scission by a physical mechanism that may function independently from or in synergy with pinchase activity.PaperFlick Help with MOV filesOptionsDownload video (32745 K)
Graphical AbstractFigure optionsDownload high-quality image (426 K)Download as PowerPoint slideHighlights
► Shiga toxin-induced membrane tubules can undergo spontaneous scission
► Membrane reorganization and domain formation correlate with scission
► Scission results from domain boundary forces and does not require dynamin activity
► Actin polymerization triggers scission by inducing membrane reorganization
Journal: - Volume 140, Issue 4, 19 February 2010, Pages 540–553