کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2036699 1072276 2010 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two Cyclin-Dependent Kinase Pathways Are Essential for Polarized Trafficking of Presynaptic Components
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Two Cyclin-Dependent Kinase Pathways Are Essential for Polarized Trafficking of Presynaptic Components
چکیده انگلیسی

SummaryPolarized trafficking of synaptic proteins to axons and dendrites is crucial to neuronal function. Through forward genetic analysis in C. elegans, we identified a cyclin (CYY-1) and a cyclin-dependent Pctaire kinase (PCT-1) necessary for targeting presynaptic components to the axon. Another cyclin-dependent kinase, CDK-5, and its activator p35, act in parallel to and partially redundantly with the CYY-1/PCT-1 pathway. Synaptic vesicles and active zone proteins mostly mislocalize to dendrites in animals defective for both PCT-1 and CDK-5 pathways. Unlike the kinesin-3 motor, unc-104/Kif1a mutant, cyy-1 cdk-5 double mutants have no reduction in anterogradely moving synaptic vesicle precursors (SVPs) as observed by dynamic imaging. Instead, the number of retrogradely moving SVPs is dramatically increased. Furthermore, this mislocalization defect is suppressed by disrupting the retrograde motor, the cytoplasmic dynein complex. Thus, PCT-1 and CDK-5 pathways direct polarized trafficking of presynaptic components by inhibiting dynein-mediated retrograde transport and setting the balance between anterograde and retrograde motors.

Graphical AbstractFigure optionsDownload high-quality image (313 K)Download as PowerPoint slideHighlights
► C. elegans cyclin CYY-1 activates CDK-5-related kinase PCT-1
► PCT-1 and CDK-5 parallel pathways localize presynaptic molecules to axons
► The pathways suppress retrograde movements of synaptic vesicles
► This is likely due to inhibition of the cytoplasmic dynein retrograde motor complex

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 141, Issue 5, 28 May 2010, Pages 846–858
نویسندگان
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