کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2036728 1072278 2009 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hematopoietic Stem Cell Development Is Dependent on Blood Flow
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Hematopoietic Stem Cell Development Is Dependent on Blood Flow
چکیده انگلیسی

SummaryDuring vertebrate embryogenesis, hematopoietic stem cells (HSCs) arise in the aorta-gonads-mesonephros (AGM) region. We report here that blood flow is a conserved regulator of HSC formation. In zebrafish, chemical blood flow modulators regulated HSC development, and silent heart (sih) embryos, lacking a heartbeat and blood circulation, exhibited severely reduced HSCs. Flow-modifying compounds primarily affected HSC induction after the onset of heartbeat; however, nitric oxide (NO) donors regulated HSC number even when treatment occurred before the initiation of circulation, and rescued HSCs in sih mutants. Morpholino knockdown of nos1 (nnos/enos) blocked HSC development, and its requirement was shown to be cell autonomous. In the mouse, Nos3 (eNos) was expressed in HSCs in the AGM. Intrauterine Nos inhibition or embryonic Nos3 deficiency resulted in a reduction of hematopoietic clusters and transplantable murine HSCs. This work links blood flow to AGM hematopoiesis and identifies NO as a conserved downstream regulator of HSC development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 137, Issue 4, 15 May 2009, Pages 736–748
نویسندگان
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