کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2036802 1072283 2009 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Signaling Principle for the Specification of the Germ Cell Lineage in Mice
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
A Signaling Principle for the Specification of the Germ Cell Lineage in Mice
چکیده انگلیسی

SummarySpecification of the germ cell lineage is vital to development and heredity. In mice, the germ cell fate is induced in pluripotent epiblast cells by signaling molecules, yet the underlying mechanism remains unknown. Here we demonstrate that germ cell fate in the epiblast is a direct consequence of Bmp4 signaling from the extraembryonic ectoderm (ExE), which is antagonized by the anterior visceral endoderm (AVE). Strikingly, Bmp8b from the ExE restricts AVE development, thereby contributing to Bmp4 signaling. Furthermore, Wnt3 in the epiblast ensures its responsiveness to Bmp4. Serum-free, defined cultures revealed that, in response to Bmp4, competent epiblast cells uniformly expressed key transcriptional regulators Blimp1 and Prdm14 and acquired germ-cell properties, including genome-wide epigenetic reprogramming, in an orderly fashion. Notably, the induced cells contributed to both spermatogenesis and fertility of offspring. By identifying a signaling principle in germ cell specification, our study establishes a robust strategy for reconstituting the mammalian germ cell lineage in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 137, Issue 3, 1 May 2009, Pages 571–584
نویسندگان
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