کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2036844 1072285 2010 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Structural Basis for Mechanical Force Regulation of the Adhesin FimH via Finger Trap-like β Sheet Twisting
چکیده انگلیسی

SummaryThe Escherichia coli fimbrial adhesive protein, FimH, mediates shear-dependent binding to mannosylated surfaces via force-enhanced allosteric catch bonds, but the underlying structural mechanism was previously unknown. Here we present the crystal structure of FimH incorporated into the multiprotein fimbrial tip, where the anchoring (pilin) domain of FimH interacts with the mannose-binding (lectin) domain and causes a twist in the β sandwich fold of the latter. This loosens the mannose-binding pocket on the opposite end of the lectin domain, resulting in an inactive low-affinity state of the adhesin. The autoinhibition effect of the pilin domain is removed by application of tensile force across the bond, which separates the domains and causes the lectin domain to untwist and clamp tightly around the ligand like a finger-trap toy. Thus, β sandwich domains, which are common in multidomain proteins exposed to tensile force in vivo, can undergo drastic allosteric changes and be subjected to mechanical regulation.

Graphical AbstractFigure optionsDownload high-quality image (122 K)Download as PowerPoint slideHighlights
► Bacterial adhesin FimH crystal structure reveals basis of force-enhanced function
► Interaction of FimH anchoring- and mannose-binding domains induces β sheet twisting
► Force-induced untwisting in the mannose-binding domain of FimH acts like a finger trap
► The finger trap enables FimH to bind mannose more strongly via catch bonds

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 141, Issue 4, 14 May 2010, Pages 645–655
نویسندگان
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