کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2037201 1072303 2008 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of a Translational Profiling Approach for the Comparative Analysis of CNS Cell Types
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Application of a Translational Profiling Approach for the Comparative Analysis of CNS Cell Types
چکیده انگلیسی

SummaryComparative analysis can provide important insights into complex biological systems. As demonstrated in the accompanying paper, translating ribosome affinity purification (TRAP) permits comprehensive studies of translated mRNAs in genetically defined cell populations after physiological perturbations. To establish the generality of this approach, we present translational profiles for 24 CNS cell populations and identify known cell-specific and enriched transcripts for each population. We report thousands of cell-specific mRNAs that were not detected in whole-tissue microarray studies and provide examples that demonstrate the benefits deriving from comparative analysis. To provide a foundation for further biological and in silico studies, we provide a resource of 16 transgenic mouse lines, their corresponding anatomic characterization, and translational profiles for cell types from a variety of central nervous system structures. This resource will enable a wide spectrum of molecular and mechanistic studies of both well-known and previously uncharacterized neural cell populations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 135, Issue 4, 14 November 2008, Pages 749–762
نویسندگان
, , , , , , , , , , , , ,