کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2037816 1072338 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression
چکیده انگلیسی

SummaryThe cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 132, Issue 3, 8 February 2008, Pages 487–498
نویسندگان
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