کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2037841 1072339 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Capture of Authentic Embryonic Stem Cells from Rat Blastocysts
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Capture of Authentic Embryonic Stem Cells from Rat Blastocysts
چکیده انگلیسی

SummaryEmbryonic stem (ES) cells have been available from inbred mice since 1981 but have not been validated for other rodents. Failure to establish ES cells from a range of mammals challenges the identity of cultivated stem cells and our understanding of the pluripotent state. Here we investigated derivation of ES cells from the rat. We applied molecularly defined conditions designed to shield the ground state of authentic pluripotency from inductive differentiation stimuli. Undifferentiated cell lines developed that exhibited diagnostic features of ES cells including colonization of multiple tissues in viable chimeras. Definitive ES cell status was established by transmission of the cell line genome to offspring. Derivation of germline-competent ES cells from the rat paves the way to targeted genetic manipulation in this valuable biomedical model species. Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 135, Issue 7, 26 December 2008, Pages 1287–1298
نویسندگان
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