کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2037842 1072339 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts
چکیده انگلیسی

SummaryRats have important advantages over mice as an experimental system for physiological and pharmacological investigations. The lack of rat embryonic stem (ES) cells has restricted the availability of transgenic technologies to create genetic models in this species. Here, we show that rat ES cells can be efficiently derived, propagated, and genetically manipulated in the presence of small molecules that specifically inhibit GSK3, MEK, and FGF receptor tyrosine kinases. These rat ES cells express pluripotency markers and retain the capacity to differentiate into derivatives of all three germ layers. Most importantly, they can produce high rates of chimerism when reintroduced into early stage embryos and can transmit through the germline. Establishment of authentic rat ES cells will make possible sophisticated genetic manipulation to create models for the study of human diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 135, Issue 7, 26 December 2008, Pages 1299–1310
نویسندگان
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