کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2037911 | 1072342 | 2008 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: MazF, an mRNA Interferase, Mediates Programmed Cell Death during Multicellular Myxococcus Development MazF, an mRNA Interferase, Mediates Programmed Cell Death during Multicellular Myxococcus Development](/preview/png/2037911.png)
SummaryIn prokaryotes, the toxin-antitoxin systems are thought to play important roles in growth regulation under stress conditions. In the E. coli MazE-MazF system, MazF toxin functions as an mRNA interferase cleaving mRNAs at ACA sequences to inhibit protein synthesis leading to cell growth arrest. Myxococcus xanthus is a bacterium displaying multicellular fruiting body development during which approximately 80% of cells undergo obligatory cell lysis. Here, we demonstrate that M. xanthus has a solitary mazF gene that lacks a cotranscribed antitoxin gene. The mazF deletion results in elimination of the obligatory cell death during development causing dramatic reduction in spore formation. Surprisingly, MrpC, a key developmental regulator, functions as a MazF antitoxin and a mazF transcription activator. Transcription of mrpC and mazF is negatively regulated via MrpC phosphorylation by a Ser/Thr kinase cascade. These findings reveal the regulated deployment of a toxin gene for developmental programmed cell death in bacteria.
Journal: - Volume 132, Issue 1, 11 January 2008, Pages 55–66