کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2038561 1072378 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Proline Isomerization of Histone H3 Regulates Lysine Methylation and Gene Expression
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Proline Isomerization of Histone H3 Regulates Lysine Methylation and Gene Expression
چکیده انگلیسی

SummaryThe cis-trans isomerization of proline serves as a regulatory switch in signaling pathways. We identify the proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-terminal tail of histones H3 and H4 and catalyses the isomerization of H3 proline P30 and P38 in vitro. We show that P38 is necessary for methylation of K36 and that isomerization by Fpr4 inhibits the ability of Set2 to methylate H3 K36 in vitro. These results suggest that the conformational state of P38, controlled by Fpr4, is important for methylation of H3K36 by Set2. Consistent with such an antagonistic role, abrogation of Fpr4 catalytic activity in vivo results in increased levels of H3K36 methylation and delayed transcriptional induction kinetics of specific genes in yeast. These results identify proline isomerization as a novel noncovalent histone modification that regulates transcription and provides evidence for crosstalk between histone lysine methylation and proline isomerization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 126, Issue 5, 8 September 2006, Pages 905–916
نویسندگان
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