کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2038770 1072393 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal Structure of the CSL-Notch-Mastermind Ternary Complex Bound to DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Crystal Structure of the CSL-Notch-Mastermind Ternary Complex Bound to DNA
چکیده انگلیسی

SummaryNotch signaling mediates communication between cells and is essential for proper embryonic patterning and development. CSL is a DNA binding transcription factor that regulates transcription of Notch target genes by interacting with coregulators. Transcriptional activation requires the displacement of corepressors from CSL by the intracellular portion of the receptor Notch (NotchIC) and the recruitment of the coactivator protein Mastermind to the complex. Here we report the 3.1 Å structure of the ternary complex formed by CSL, NotchIC, and Mastermind bound to DNA. As expected, the RAM domain of Notch interacts with the β trefoil domain of CSL; however, the C-terminal domain of CSL has an unanticipated central role in the interface formed with the Notch ankyrin repeats and Mastermind. Ternary complex formation induces a substantial conformational change within CSL, suggesting a molecular mechanism for the conversion of CSL from a repressor to an activator.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 124, Issue 5, 10 March 2006, Pages 985–996
نویسندگان
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