کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039779 1073081 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimal ROS Signaling Is Critical for Nuclear Reprogramming
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Optimal ROS Signaling Is Critical for Nuclear Reprogramming
چکیده انگلیسی


• Optimal ROS signaling is required in the early stages of nuclear reprogramming
• Antagonism of ROS signaling in early reprogramming suppresses iPSC generation
• Anti-oxidant enzymes increase in the late phase of reprogramming

SummaryEfficient nuclear reprogramming of somatic cells to pluripotency requires activation of innate immunity. Because innate immune activation triggers reactive oxygen species (ROS) signaling, we sought to determine whether there was a role of ROS signaling in nuclear reprogramming. We examined ROS production during the reprogramming of doxycycline (dox)-inducible mouse embryonic fibroblasts (MEFs) carrying the Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc [OSKM]) into induced pluripotent stem cells (iPSCs). ROS generation was substantially increased with the onset of reprogramming. Depletion of ROS via antioxidants or Nox inhibitors substantially decreased reprogramming efficiency. Similarly, both knockdown and knockout of p22phox—a critical subunit of the Nox (1–4) complex—decreased reprogramming efficiency. However, excessive ROS generation using genetic and pharmacological approaches also impaired reprogramming. Overall, our data indicate that ROS signaling is activated early with nuclear reprogramming, and optimal levels of ROS signaling are essential to induce pluripotency.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 15, Issue 5, 3 May 2016, Pages 919–925
نویسندگان
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