کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2040013 1073094 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polar Ejection Forces Promote the Conversion from Lateral to End-on Kinetochore-Microtubule Attachments on Mono-oriented Chromosomes
ترجمه فارسی عنوان
نیروهای تخلیه قطبی از تغییرات جانبی تا انتهای پیوند کینتوچور-میکروتوبول بر روی کروموزومهای مونو گرا
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• Spindle assembly checkpoint (SAC) can be satisfied after a delay in cells with mono-oriented chromosomes
• Mono-oriented chromosomes experience intra-kinetochore stretch
• Polar ejection forces promote SAC satisfaction independently of bi-orientation
• Polar ejection forces promote the conversion from lateral to end-on attachments

SummaryChromosome bi-orientation occurs after conversion of initial lateral attachments between kinetochores and spindle microtubules into stable end-on attachments near the cell equator. After bi-orientation, chromosomes experience tension from spindle forces, which plays a key role in the stabilization of correct kinetochore-microtubule attachments. However, how end-on kinetochore-microtubule attachments are first stabilized in the absence of tension remains a key unanswered question. To address this, we generated Drosophila S2 cells undergoing mitosis with unreplicated genomes (SMUGs). SMUGs retained single condensed chromatids that attached laterally to spindle microtubules. Over time, laterally attached kinetochores converted into end-on attachments and experienced intra-kinetochore stretch/structural deformation, and SMUGs eventually exited a delayed mitosis with mono-oriented chromosomes after satisfying the spindle-assembly checkpoint (SAC). Polar ejection forces (PEFs) generated by Chromokinesins promoted the conversion from lateral to end-on kinetochore-microtubule attachments that satisfied the SAC in SMUGs. Thus, PEFs convert lateral to stable end-on kinetochore-microtubule attachments, independently of chromosome bi-orientation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 13, Issue 3, 20 October 2015, Pages 460–468
نویسندگان
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