کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047635 | 1074006 | 2015 | 5 صفحه PDF | دانلود رایگان |
• VAMP-2 was identified as a target of miR-206.
• miR-206 decreased the expression of VAMP-2 protein.
• miR-206 reduced lung surfactant secretion in alveolar type II cells.
Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3′-untranslational region (3′-UTR) luciferase assay. Mutations in the predicated miR-206 binding sites reduced the binding of miR-206 to the 3′-UTR of VAMP-2. miR-206 decreased the expression of VAMP-2 protein and decreased the lung surfactant secretion in alveolar type II cells. In conclusion, miR-206 regulates lung surfactant secretion by limiting the availability of VAMP-2 protein.
Journal: FEBS Letters - Volume 589, Issue 1, 2 January 2015, Pages 172–176