کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047654 1074011 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Processive cytoskeletal motors studied with single-molecule fluorescence techniques
ترجمه فارسی عنوان
موتورهای سیتو اسکلتیو پروسوی مورد مطالعه با تکنیک های تک مولکولی فلورسانس مورد مطالعه قرار گرفتند
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
چکیده انگلیسی

Processive cytoskeletal motors from the myosin, kinesin, and dynein families walk on actin filaments and microtubules to drive cellular transport and organization in eukaryotic cells. These remarkable molecular machines are able to take hundreds of successive steps at speeds of up to several microns per second, allowing them to effectively move vesicles and organelles throughout the cytoplasm. Here, we focus on single-molecule fluorescence techniques and discuss their wide-ranging applications to the field of cytoskeletal motor research. We cover both traditional fluorescence and sub-diffraction imaging of motors, providing examples of how fluorescence data can be used to measure biophysical parameters of motors such as coordination, stepping mechanism, gating, and processivity. We also outline some remaining challenges in the field and suggest future directions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 19, 1 October 2014, Pages 3520–3525
نویسندگان
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