کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047726 | 1074016 | 2014 | 10 صفحه PDF | دانلود رایگان |

• BjAnn3 protects Saccharomyces cerevisiae cells from oxidative stress.
• BjAnn3 interferes with antioxidant gene expression for improved cellular-protection.
• BjAnn3 counteracts the loss of thiol-specific antioxidant function across kingdom.
Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H2O2. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins.
Journal: FEBS Letters - Volume 588, Issue 4, 14 February 2014, Pages 584–593