کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047846 1074037 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
FixK2, a key regulator in Bradyrhizobium japonicum, is a substrate for the protease ClpAP in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
FixK2, a key regulator in Bradyrhizobium japonicum, is a substrate for the protease ClpAP in vitro
چکیده انگلیسی

FixK2 is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP1 and ClpXP1 were purified and tested. FixK2 was found to be degraded by ClpAP1 but not by ClpXP1. Degradation was inhibited by the ClpS1 adaptor protein, indicating that FixK2 is a direct substrate for ClpAP1. The last 12 amino acids of FixK2 appeared to be recognized by ClpA. The results suggest that the ClpAP system is involved in the cellular turnover of FixK2.


► Purification and characterization of Clp proteins from Bradyrhizobium japonicum.
► ClpA of B. japonicum is autodegraded in a ClpAP-dependent manner.
► FixK2, a key regulatory protein, is specifically degraded by ClpAP1.
► The C-terminus of FixK2 is the main recognition site for ClpA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 1, 4 January 2013, Pages 88–93
نویسندگان
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