کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047860 1074039 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
BM61 of Bombyx mori nucleopolyhedrovirus: Its involvement in the egress of nucleocapsids from the nucleus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
BM61 of Bombyx mori nucleopolyhedrovirus: Its involvement in the egress of nucleocapsids from the nucleus
چکیده انگلیسی

All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm.


► We constructed a Bm61 knockout virus.
► We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus.
► We report that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 586, Issue 7, 5 April 2012, Pages 990–995
نویسندگان
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