کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047907 1074045 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Slow growth and unstable ribosomal RNA lacking pseudouridine in mouse embryonic fibroblast cells expressing catalytically inactive dyskerin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Slow growth and unstable ribosomal RNA lacking pseudouridine in mouse embryonic fibroblast cells expressing catalytically inactive dyskerin
چکیده انگلیسی


• MEF cells with a mutant dyskerin have no detectable pseudouridine in ribosomal RNA.
• Catalytically null mutant dyskerin D125A is very unstable.
• Pseudouridine is important in rRNA processing in IVS1.
• Pseudouridine is important for ribosomal RNA stability.
• Pseudouridine stabilizes the functional secondary structure of RNA.

Pseudouridine is the most abundant modified nucleotide in ribosomal RNA throughout eukaryotes and archaea but its role is not known. Here we produced mouse embryonic fibroblast cells expressing only catalytically inactive dyskerin, the pseudouridine synthase that converts uridine to pseudouridine in ribosomal RNA. The mutant dyskerin protein, D125A, was extremely unstable but cells were able to divide and grow very slowly. Abnormalities in ribosome RNA synthesis were apparent but mature cytoplasmic RNAs lacking pseudouridine were produced and were very unstable. We conclude that pseudouridine is required to stabilize the secondary structure of ribosomal RNA that is essential for its function.

Structured summary of protein interactions:fibrillarin and Dkc1colocalize by fluorescence microscopy (View interaction)

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 14, 11 July 2013, Pages 2112–2117
نویسندگان
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