کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047928 1074045 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recognition of intermolecular G-quadruplexes by full length nucleophosmin. Effect of a leukaemia-associated mutation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Recognition of intermolecular G-quadruplexes by full length nucleophosmin. Effect of a leukaemia-associated mutation
چکیده انگلیسی

Nucleophosmin (NPM) is a nucleolar protein involved in ribosome biogenesis. NPM1 gene is frequently mutated in acute myeloid leukaemia (AML), correlating with aberrant cytoplasmic localization of the protein. NPM attachment to the nucleolus in physiological conditions probably depends on binding to nucleic acids, and this recognition could be altered in AML. NPM associates to guanine-rich DNA sequences, able to fold as “G-quadruplexes”. We have analyzed the interaction of pentameric, full length NPM with G-rich oligonucleotides, finding that the protein binds preferentially high-order G-quadruplexes. AML-associated mutation significantly hampers DNA binding, pointing to a possible mechanism contributing to pathological mislocalization of NPM.


• Full length nucleophosmin is able to recognize oligomeric assemblies of G-rich DNA.
• Nucleophosmin interaction with DNA involves multiple binding sites in the protein.
• AML-associated mutation of nucleophosmin severely hampers its DNA binding ability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 14, 11 July 2013, Pages 2254–2259
نویسندگان
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