کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2048197 | 1074069 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Methyl-Typing: An improved and visualized COBRA software for epigenomic studies Methyl-Typing: An improved and visualized COBRA software for epigenomic studies](/preview/png/2048197.png)
Combined bisulfite restriction analysis (COBRA) is one of the most commonly used methylation quantification methods. However, it focuses on relatively few restriction enzymes. Here, we present Methyl-Typing, a web-based software that provides restriction enzyme mining data for methyl-cytosine-containing sequences following bisulfite-conversion. Gene names, accession numbers, sequences, PCR primers, and file upload are accessible for input. Promoter sequences and restriction enzymes for CpG- and GpC-containing recognition sites are retrieved. Four representative enzymes were tested successfully by COBRA on the experimental work. Therefore, the Methyl-Typing tool provides a comprehensive COBRA-restriction enzyme mining. It is freely available at http://bio.kuas.edu.tw/methyl-typing.
Journal: FEBS Letters - Volume 584, Issue 4, 19 February 2010, Pages 739–744