کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048323 | 1074076 | 2012 | 6 صفحه PDF | دانلود رایگان |
In filamentous fungi d-galactose can be catabolised through the oxido-reductive and/or the Leloir pathway. In the oxido-reductive pathway d-galactose is converted to d-fructose in a series of steps where the last step is the oxidation of d-sorbitol by an NAD-dependent dehydrogenase. We identified a sorbitol dehydrogenase gene, sdhA (JGI53356), in Aspergillus niger encoding a medium chain dehydrogenase which is involved in d-galactose and d-sorbitol catabolism. The gene is upregulated in the presence of d-galactose, galactitol and d-sorbitol. An sdhA deletion strain showed reduced growth on galactitol and growth on d-sorbitol was completely abolished. The purified enzyme converted d-sorbitol to d-fructose with Km of 50 ± 5 mM and vmax of 80 ± 10 U/mg.
► We identified the sorbitol dehydrogenase gene in Aspergillusniger.
► It is part of the oxido-reductive d-galactose pathway.
► It is upregulated on d-sorbitol, d-galactose and galactitol.
Journal: FEBS Letters - Volume 586, Issue 4, 17 February 2012, Pages 378–383