کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048325 | 1074076 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression](/preview/png/2048325.png)
It has been reported that expression of glucose transporter member 3 (GLUT3) is up-regulated in bladder cancers. However, the regulating mechanism remains unknown. Here, we assessed whether microRNAs (miRNAs) regulate GLUT3 expression in bladder cancers. In our study, miR-195-5p was identified to directly targeted GLUT3 3′-untranslated region (UTR) in bladder cancer T24 cells. Small interfering RNA (siRNA)- and miR-195-5p-mediated GLUT3 knockdown experiments revealed that miR-195-5p decreased T24 cells glucose uptake, inhibited cell growth and promoted cell apoptosis through suppression of GLUT3 expression. Therefore, miR-195-5p is a novel and also the first identified miRNA that targets GLUT3, and the aberrant decreased expression of miR-195-5p and consequent GLUT3 up-regulation may contribute to bladder carcinogenesis.
► miR-195-5p negatively regulates the expression of GLUT3 through GLUT3 3’UTR.
► miR-195-5p is the first identified microRNA that targets GLUT3.
► Over-expression of miR-195-5p decreases GLUT3-mediated glucose uptake.
► miR-195-5p significantly inhibits cell growth through reducing GLUT3 expression.
Journal: FEBS Letters - Volume 586, Issue 4, 17 February 2012, Pages 392–397