کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048327 | 1074076 | 2012 | 8 صفحه PDF | دانلود رایگان |
In this study, we identified a variable region in the C-terminus of hemocyanin from the shrimp Litopenaeus vannamei (2288–2503 bp, HcSC) by sequence alignments. A total of 13 SNPs were identified by PCR-SSCP and HcSC clone sequencing. The SSCP patterns of HcSC could be modulated in Vibro parahaemolyticus-treated shrimps. A novel SSCP band with four SNP sites was identified in V. parahaemolyticus-resistant shrimps. More importantly, three of these four SNPs introduced variations in amino acid sequence and possibly secondary structure of the HcSC polypeptide and resulted in a higher agglutinative activity against seven pathogenic bacteria. These results suggest that the C-terminus of shrimp L. vannamei hemocyanin possesses SNPs, which may be related to shrimp resistance to different pathogens.
► We found for the first time that the C-terminus of hemocyanin possessed SNPs.
► It could be modulated by a specific environmental stress.
► Some specific SNP sites might be associated with enhanced resistance of shrimp to pathogens.
Journal: FEBS Letters - Volume 586, Issue 4, 17 February 2012, Pages 403–410