کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048446 | 1074081 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Cyclic cytidine 3′,5′-monophosphate (cCMP) signals via cGMP kinase I Cyclic cytidine 3′,5′-monophosphate (cCMP) signals via cGMP kinase I](/preview/png/2048446.png)
We analysed the function and intracellular signalling of the cyclic pyrimidinic nucleotide cCMP. The membrane-permeable cCMP analogue dibutyryl-cCMP mediated mouse aorta relaxation. cCMP activated purified cGMP-dependent protein kinase (cGK) Iα and Iβ and stimulated cGK in aorta lysates. cCMP-induced relaxation was abolished in cGKI-knockout tissue. Additionally, deletion of inositol–trisphosphate receptor associated cGKI substrate (IRAG) suppressed cCMP-mediated relaxation. Signalling of cCMP via cGKI/IRAG appears to be of broader physiological importance because cCMP-mediated inhibition of platelet aggregation was absent in cGKI- and IRAG-deficient platelets. These results demonstrate that cCMP acts as intracellular messenger molecule, most unexpectedly utilizing the cGMP signal transduction pathway.
Journal: FEBS Letters - Volume 584, Issue 18, 24 September 2010, Pages 3979–3984