کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048456 1074081 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Epitope tag-induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Epitope tag-induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase
چکیده انگلیسی

Ctf7/Eco1-dependent acetylation of Smc3 is essential for sister chromatid cohesion. Here, we use epitope tag-induced lethality in cells diminished for Ctf7/Eco1 activity to map cohesin architecture in vivo. Tagging either Smc1 or Mcd1/Scc1, but not Scc3/Irr1, appears to abolish access to Smc3 in ctf7/eco1 mutant cells, suggesting that Smc1 and Smc3 head domains are in direct contact with each other and also with Mcd1/Scc1. Thus, cohesin complexes may be much more compact than commonly portrayed. We further demonstrate that mutation in ELG1 or RFC5 anti-establishment genes suppress tag-induced lethality, consistent with the notion that the replication fork regulates Ctf7/Eco1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 18, 24 September 2010, Pages 4037–4040
نویسندگان
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