کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048598 1074084 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature
چکیده انگلیسی

We performed in vivo phage display in the stroke prone spontaneously hypertensive rat, a cardiovascular disease model, and the normotensive Wistar Kyoto rat to identify cardiac targeting peptides, and then assessed each in the context of viral gene delivery. We identified both common and strain-selective peptides, potentially indicating ubiquitous markers and those found selectively in dysfunctional microvasculature of the heart. We show the utility of the peptide, DDTRHWG, for targeted gene delivery in human cells and rats in vivo when cloned into the fiber protein of subgroup D adenovirus 19p. This study therefore identifies cardiac targeting peptides by in vivo phage display and the potential of a candidate peptide for vector targeting strategies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 12, 18 June 2009, Pages 2100–2107
نویسندگان
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