کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048622 | 1074086 | 2011 | 8 صفحه PDF | دانلود رایگان |

It has become increasingly clear that microRNAs play an important role in many human diseases including cancer. Here, we show that expression of miR-21 in HEK293 and several colorectal cancer cells was found inversely correlated with ras homolog gene family, member B (RhoB) expression. miR-21 expression significantly suppressed RhoB 3′ UTR luciferase-reporter activity, but the inhibitory effect was lost when the putative target sites were mutated. Exogenous miR-21 over-expression mimicked the effect of RhoB knockdown in promoting proliferation and invasion and inhibiting apoptosis, whereas anti-miR-21 or RhoB expression yielded opposite effects, in colorectal cancer cells. These results suggest that miR-21 is a regulator of RhoB expression and RhoB could be a useful target in exploring the potential therapeutic benefits of miR-21 mediated tumor cell behaviors in colorectal cancer.
► miR-21 targets the tumor suppressor RhoB in colorectal cancer cells.
► Expression of miR-21 in HEK293 and several colorectal cancer cells was found inversely correlated with RhoB expression.
► miR-21-RhoB signaling axis regulates proliferation, invasion and apoptosis in colorectal cancer cells.
Journal: FEBS Letters - Volume 585, Issue 19, 3 October 2011, Pages 2998–3005