Mapping of interaction domains of putative telomere-binding proteins AtTRB1 and AtPOT1b from Arabidopsis thaliana

We previously searched for interactions between plant telomere-binding proteins and found that AtTRB1, from the single-myb-histone (Smh) family, interacts with the Arabidopsis POT1-like-protein, AtPOT1b, involved in telomere capping. Here we identify domains responsible for that interaction. We also map domains in AtTRB1 responsible for interactions with other Smh-family-members. Our results show that the N-terminal OB-fold-domain of AtPOT1b mediates the interaction with AtTRB1. This domain is characteristic for POT1- proteins and is involved with binding the G-rich-strand of telomeric DNA. AtPOT1b also interacts with AtTRB2 and AtTRB3. The central histone-globular-domain of AtTRB1 is involved with binding to AtTRB2 and 3, as well as to AtPOT1b. AtTRB1-heterodimers with other Smh-family-members are more stable than AtTRB1-homodimers. Our results reveal interaction networks of plant telomeres.Structured summaryMINT-6440051:AtTRB1 (uniprotkb:Q8VWK4) physically interacts (MI:0218) with AtTRB1 (uniprotkb:Q8VWK4) by two-hybrid (MI:0018)MINT-6440068:AtTRB2 (uniprotkb:Q8VX38) physically interacts (MI:0218) with AtTRB1 (uniprotkb:Q8VWK4) by two-hybrid (MI:0018)MINT-6440083:AtTRB3 (uniprotkb:Q9M2X3) physically interacts (MI:0218) with AtTRB1 (uniprotkb:Q8VWK4) by two-hybrid (MI:0018)MINT-6440099:AtPOT1b (uniprotkb:Q6Q835) physically interacts (MI:0218) with AtTRB1 (uniprotkb:Q8VWK4) by two-hybrid (MI:0018)MINT-6440119:AtPOT1b (uniprotkb:Q6Q835) physically interacts (MI:0218) with AtTRB2 (uniprotkb:Q8VX38) by two-hybrid (MI:0018)MINT-6440138:AtPOT1b (uniprotkb:Q6Q835) physically interacts (MI:0218) with AtTRB3 (uniprotkb:Q9M2X3) by two-hybrid (MI:0018)MINT-6440216:AtPOT1b (uniprotkb:Q6Q835) physically interacts (MI:0218) with AtTRB1 (uniprotkb:Q8VWK4) by coimmunoprecipitation (MI:0019)MINT-6440157:AtTRB2 (uniprotkb:Q8VX38) physically interacts (MI:0218) with AtTRB1 (uniprotkb:Q8VWK4) by coimmunoprecipitation (MI:0019)MINT-6440177:AtTRB3 (uniprotkb:Q9M2X3) physically interacts (MI:0218) with AtTRB1 (uniprotkb:Q8VWK4) by coimmunoprecipitation (MI:0019)