کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2049403 | 1074127 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The ND2 subunit is labeled by a photoaffinity analogue of asimicin, a potent complex I inhibitor The ND2 subunit is labeled by a photoaffinity analogue of asimicin, a potent complex I inhibitor](/preview/png/2049403.png)
NADH:ubiquinone oxidoreductase (complex I) is the entry enzyme of mitochondrial oxidative phosphorylation. To obtain the structural information on inhibitor/quinone binding sites, we synthesized [3H]benzophenone-asimicin ([3H]BPA), a photoaffinity analogue of asimicin, which belongs to the acetogenin family known as the most potent complex I inhibitor. We found that [3H]BPA was photo-crosslinked to ND2, ND1 and ND5 subunits, by the three dimensional separation (blue-native/doubled SDS–PAGE) of [3H]BPA-treated bovine heart submitochondrial particles. The cross-linking was blocked by rotenone. This is the first finding that ND2 was photo-crosslinked with a potent complex I inhibitor, suggesting its involvement in the inhibitor/quinone-binding.
Journal: FEBS Letters - Volume 584, Issue 5, 5 March 2010, Pages 883–888